HPLC Columns and Cartridges
Mac-Mod Evosphere-BIO INERT C18/AR, 300 Å, 5 µm, 4 x 250 mm Monodisperse Fully Porous Particle Column in Inert-Coated Hardware
Evosphere UHPLC and HPLC columns are 100 A Monodispersed Fully Porous Silica-based columns designed to combine the benefits of Fully Porous and Core-Shell particle technology into an improved particle design and morphology that brings cutting-edge loading capacity to ultra-high-resolution particle columns. The Monodispersed Particles provide similar efficiency to Core-Shell products when comparing them directly via particle size matching and significantly increase loadability which allows ultra-high resolution analytical separations to scale to preparative separations for isolation work if needed. The bonded phase chemistries are exciting as well to maximize selectivity with novel C12 bonded phase, 2 multi-modal C18/AR, and C18/PFP bonded phases, an extended and stabilized phenyl funtionality in the Diphenyl bonded phase, a polar endcapped AQUA phase, a Phenyl-Hexyl phase, a propyl PFP phase and the RP18-Amide bonded phases which is great for doing bases at low pH.
Waters Corp XBridge Glycan BEH Amide Column, 2.5 μm, 130 Å, 2.1 x 100 mm
The Waters XBridge Glycan BEH Amide Column is a high-performance HPLC column for robust and reproducible separations of glycans and other highly polar compounds. It uses BEH particle technology and a stable amide phase for HILIC applications, ensuring consistency and transferability between HPLC and UPLC platforms.
- Ensures consistent glycan profiles and batch uniformity.
- Stable BEH Amide chemistry for HILIC separations.
- Effectively separates and retains highly polar compounds.
- Facilitates separation transfer between HPLC and UPLC.
- Maintains stability over a broad pH range.
- Designed for HILIC separations of 2-AB, 2-AA, or RapiFluor-MS™ labeled glycans.
- Recommended operating pH range: 3 to 8.
- Recommended operating temperatures: 20°C to 90°C.
Waters Corporation C18 Column, 1.7 μm, 2.1 x 50 mm
These Peptide Separation Technology Columns are optimized and QC tested for peptide separations. With a 130Å small-pore BEH particle, they offer a wide pH range, superior low pH stability, and ultra-low column bleed. Suitable for UPLC, UHPLC, and LC-MS methods, these columns enhance reproducibility, peak shape, and recovery for metal-sensitive analytes.
- Optimized and QC tested for peptide separations.
- Features a 130Å small-pore BEH particle, offering wide pH range, superior low pH stability, and ultra-low column bleed.
- Utilizes MaxPeak High Performance Surfaces (HPS) technology, enhancing reproducibility and peak shape for metal-sensitive analytes.
- Reduces column conditioning and passivation times, enabling simpler mobile phases with improved sensitivity.
- Designed for peptide analysis, providing outstanding peak capacity superior peak shape.
Waters Corp Column, C4, 300 Å, 2.5 μm, 4.6 x 150 mm
This column is optimized for separating proteins by size, hydrophobicity, and isoelectric point. Its C4 ligand offers less retention than C18 phases, minimizing protein carryover and improving recovery and peak capacity. As a MaxPeak Premier Class Column, it delivers consistent chromatographic performance, enhanced reproducibility, peak shape, and recovery for metal-sensitive analytes.
- Separates proteins of various sizes, hydrophobicities, and isoelectric points.
- QC tested with MassPREP Protein Mix standard.
- Provides consistent chromatography with enhanced reproducibility and peak shape.
- Improves recovery for metal-sensitive analytes; minimizes protein carryover.
- Tolerates extreme pH and temperature.
- Minimizes secondary interactions.
- Enhances sensitivity for phosphorylated proteins and low-level intact/subunit mAb analyses.
Waters Corp Waters XBridge Glycan BEH Amide Column, 130Å, 3.5 μm, 4.6 x 100 mm
These XBridge Glycan BEH Amide Columns are designed for Hydrophilic Interaction Liquid Chromatography (HILIC) of labeled glycans, providing stable and efficient analysis of neutral and charged species.
- Designed for HILIC separations of 2-aminobenzamide (2-AB), 2-aminobenzoic acid (2-AA), or RapiFluor-MS™ labeled glycans.
- Separates both neutral and charged labeled glycan species.
- Features chemical and mechanical stability due to ethylene bridged hybrid particle composition.
- Scalable particle sizes for UPLC (1.7 μm) and HPLC (2.5 μm XP, 3.5 μm) applications.
- Recommended operating pH range of 3 to 8.
- Operates between 20°C to 90°C, enhancing selectivity, lowering solvent viscosity, and increasing mass transfer rates.
Waters Corp Column, C18+, 90Å, 2.7 μm, 4.6 mm, 30 mm
These general purpose, charged surface, high efficiency C18 columns utilize solid-core particles to deliver excellent peak shape for basic analytes at low pH. They enable quicker separations for higher throughput, allowing more concurrent samples and reducing lab operation costs without compromising separation performance.
- Excellent peak shape for basic analytes at low pH.
- Quicker separations for higher throughput and reduced lab costs.
- Solid-core particles improve performance by optimizing the van Deemter equation terms.
- Improved sample throughput with equivalent or superior peak capacities.
- Operates across high pressure up to 18,000 psi (1240 Bar).
- Wide operating pH range from 2 to 8.
- Compatible with VanGuard cartridges for enhanced protection and extended lifetime.
Waters Corp Column, C8 Chemistry, 80 Å, 5 μm, 4.6 mm ID, 250 mm Length
Waters Column, C8 Chemistry, 80 Å, 5 μm, 4.6 mm ID, 250 mm Length
Waters Corp Waters OBD Prep Column, Diol (OH), 5 μm, 19 mm x 50 mm
Torus DIOL Columns provide additional selectivity choices. The high-density diol surface bonding offers chromatography performance similar to traditional, unbonded silica phases, and enhances overall method robustness when utilized with additives.
- Excellent peak shapes
- Wide range of unique selectivities with unique ligands
- Highest efficiency and QC-ready robustness
- OBD Technology
- Designed for achiral SFC separations
- Ideally suited for method transfer and method scale-up
- Based on patented two-stage functionalization of ethylene bridged hybrid (BEH) particles
- Initial bonding provides a hydrophilic surface that controls retention characteristics and minimizes unwanted surface interactions
- Robust chromatographic performance over the lifetime of the column
- Show good peak shapes for acidic compounds
Waters Corp Waters ACQUITY UPLC BEH Amide Method Validation Kit, 1.7 μm
This kit consists of three ACQUITY UPLC BEH Amide columns, each packed with a different batch of sorbent, designed for method validation.
- Amide chemistry for hydrophilic interaction (HILIC) separation.
- Hybrid particle substrate with spherical shape.
- Low silanol activity.
- Supports a pH range of 2 to 11.
- Withstands a maximum pressure of 18000 psi (1240 Bar).
- Particle size of 1.7 μm.
- Pore size of 130 Å.
- Inner diameter of 2.1 mm and length of 50 mm.
- Comes with 3 units per package.
- Features Parker-style endfitting.
- Utilizes BEH particle technology.
- Complies with USP Classification L68.
- Equipped with eCord for traceability.
- Suitable for UPLC and UHPLC systems.
- Has a 12% carbon load.
- Surface area of 185.
Advanced Materials Technology HALO 160 ES-C18 2.7 3.0
HALO BIOCLASS ES-C18 Peptide columns made with innovative Fused-Core particle technology are ideal for both ultrafast and ultrahigh resolution separations of peptides and polypeptides up to 20 kDa. HALO peptide columns offer comparable peak capacity to sub-2 μm non-core columns at 40–50% back pressure (2.7 μm), and 20% higher peak capacity than sub-2 μm non-core columns at comparable back pressure (2 μm). HALO 160 Å peptide columns are optimized for high performance in HPLC, UHPLC, and LCMS peptide applications.
Waters Corporation Column Kit, 1.7 μm, 3 mm, 100 mm, 4/pk
Waters Corporation Column Kit, 1.7 μm, 3 mm, 100 mm, 4/pk
Waters Corp Method Validation Kit, 2.5 μm, 2.1 x 150 mm
This method validation kit includes three columns, each packed with a different lot of material. The columns offer excellent retention for polar acidic analytes under reversed-phase conditions. The reversed-phase/anion-exchange mixed-mode chemistry is stable across a pH range of 2 to 10 and provides complementary selectivity to traditional C18 stationary phases, beneficial for methods development.
- RP/Anion exchange chemistry
- Mixed-mode separation
- Particle size: 2.5 μm
- Inner diameter: 2.1 mm
- Length: 150 mm
- pH range: 2 to 10
- Maximum pressure: 18000 psi (1240 Bar)
- BEH particle technology
- C18 AX bonding technology
- Low silanol activity
- Spherical particle shape
- Pore diameter: 95 Å
- System: UPLC, UHPLC
- Technique: LC
Waters Corp XBridge Peptide BEH C18 Column, 300Å, 10 μm, 50 mm x 250 mm
The XBridge Peptide BEH C18 OBD Prep Column is designed for peptide separations, optimized and QC tested with cytochrome c digest standards. It utilizes a wide-pore (300Å) trifunctionally bonded BEH particle, providing a wide usable pH range, superior low pH stability, and ultra-low column bleed. Ideal for assaying samples with large peptides in proteomics, protein characterization, and peptide synthesis.
- Optimized for peptide separations and QC tested with cytochrome c digest standards.
- Features a wide-pore (300Å) trifunctionally bonded BEH particle.
- Offers a wide usable pH range (1 to 12) with superior low pH stability and ultra-low column bleed.
- Ideal for assaying large peptides in proteomics, protein characterization, and peptide synthesis.
- Enables direct scaling from UPLC, UHPLC, or HPLC screening to lab-scale purification.